Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches

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Li, David and Chen, Yu; (2015) Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. SPIE, DEU. ISBN 9781628417012 (https://doi.org/10.1117/12.2183082)

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Abstract

A newly developed hardware-friendly non-iterative fluorescence lifetime imaging (FLIM) analysis method was verified in an FPGA chip. Its performances were also demonstrated on two-photon FLIM images of gold nanorods (GNRs)-Cy5 labelled Hela cells. The results obtained by the proposed method can be presented in a polor plot to be compared to the widely used phasor (Phasor) approach. Combining our method with Phasor will be very useful in FLIM analysis.

ORCID iDs

Li, David ORCID logoORCID: https://orcid.org/0000-0002-6401-4263 and Chen, Yu;
CORE (COnnecting REpositories)