Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator

Trägårdh, Johanna and Robb, Gillian and Gadalla, Kamal K.E. and Cobb, Stuart and Travis, Christopher and Oppo, Gian-Luca and McConnell, Gail (2015) Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator. Optics Letters. ISSN 0146-9592 (https://doi.org/10.1364/OL.40.003484)

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Abstract

We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .

ORCID iDs

Trägårdh, Johanna ORCID logoORCID: https://orcid.org/0000-0002-8662-9509, Robb, Gillian, Gadalla, Kamal K.E., Cobb, Stuart, Travis, Christopher ORCID logoORCID: https://orcid.org/0000-0002-3358-3420, Oppo, Gian-Luca ORCID logoORCID: https://orcid.org/0000-0002-5376-4309 and McConnell, Gail ORCID logoORCID: https://orcid.org/0000-0002-7213-0686;
  • Item type:Article
    ID code:53582
    Dates:
    Date
    Event
    21 July 2015
    Published
    24 June 2015
    Accepted
    Notes:© 2015 Optical Society of America. One print or electroni c copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.
    Keywords:label free imaging, optical parametric generator, wavelength, nonlinear microscopy, harmonic generation and mixing , parametric oscillators and amplifiers, Pharmacy and materia medica, Optics. Light, Atomic and Molecular Physics, and Optics
    Subjects:Medicine > Pharmacy and materia medica
    Science > Physics > Optics. Light
    Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:02 Jul 2015 14:23
    Last modified:05 Feb 2023 02:38
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/53582
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