Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator
Trägårdh, Johanna and Robb, Gillian and Gadalla, Kamal K.E. and Cobb, Stuart and Travis, Christopher and Oppo, Gian-Luca and McConnell, Gail (2015) Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator. Optics Letters. ISSN 0146-9592
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Abstract
We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .
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Trägårdh, Johanna ![]() ![]() ![]() ![]() | Item type: | Article |
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ID code: | 53582 |
Notes: | © 2015 Optical Society of America. One print or electroni c copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited. |
Keywords: | label free imaging, optical parametric generator, wavelength, nonlinear microscopy, harmonic generation and mixing , parametric oscillators and amplifiers, Pharmacy and materia medica, Optics. Light, Atomic and Molecular Physics, and Optics |
Subjects: | Medicine > Pharmacy and materia medica Science > Physics > Optics. Light |
Department: | Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Faculty of Science > Physics |
Depositing user: | Pure Administrator |
Date deposited: | 02 Jul 2015 14:23 |
Last modified: | 26 Jan 2021 02:41 |
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URI: | https://strathprints.strath.ac.uk/id/eprint/53582 |
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