Full system considerations for multiphoton microscopy
Wokosin, D.L. and Girkin, J.M.; Ammasi, Periasamy and So, Peter T.C., eds. (2001) Full system considerations for multiphoton microscopy. In: Multiphoton Microscopy in the Biomedical Sciences (Proceedings of SPIE Volume: 4262). SPIE, pp. 231-237. (http://dx.doi.org/10.1117/12.424558)
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A most-important-variables analysis of practical, successful multiphoton excitation fluorescence microscopy is presented. The key strength of multiphoton imaging -- localization of the excitation volume -- helps to decouple the excitation from the emission; the emission no longer needs to be imaged. The presentation starts with maximizing the detected signal and proceeds to the laser source considerations. The main goal is to match the instrumentation to the biological problem. The main aspects covered are... Emission collection: potential signal; Focal plane mismatch: chromatic aberration; Light detected: actual signal; Live cell imaging: preservation of biological function; Deep imaging: preservation of image contrast; Signal production: potential limitations.
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Item type: Book Section ID code: 5281 Dates: DateEventApril 2001PublishedSubjects: Science > Physics > Optics. Light Department: Faculty of Science > Physics > Institute of Photonics
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Physiology and PharmacologyDepositing user: Strathprints Administrator Date deposited: 01 Feb 2008 Last modified: 08 Apr 2024 12:21 URI: https://strathprints.strath.ac.uk/id/eprint/5281