Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy
McConnell, G. and Poland, S.P. and Girkin, J.M. (2006) Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy. Review of Scientific Instruments, 77 (013702). 013702. ISSN 0034-6748
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We report on the use of a computer-controlled digital micromirror device (DMD) to select discrete wavelength ranges from a white-light supercontinuum and the application of the light source to confocal laser scanning microscopy (CLSM). The fast switching rate of the DMD enabled video-rate wavelength tuning, while the high resolution allowed precise control over the bandwidth selection. The DMD system was used in conjunction with a CLSM system to characterize the wavelength switching capability, prior to performing three-dimensional CLSM of a variety of multiple-labeled samples in order to demonstrate the efficacy and practical application of the system.
| Author(s): | McConnell, G., Poland, S.P. and Girkin, J.M. | Item type: | Article |
|---|---|
| ID code: | 5280 |
| Keywords: | multiplexing, supercontinuum generation, micromirrors, fluorescence, optical microscopy, optical tuning, optical switches, photonics, biophotonics, Optics. Light, Instrumentation |
| Subjects: | Science > Physics > Optics. Light |
| Department: | Faculty of Science > Physics > Institute of Photonics Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
| Depositing user: | Strathprints Administrator |
| Date deposited: | 01 Feb 2008 |
| Last modified: | 31 May 2019 02:06 |
| URI: | https://strathprints.strath.ac.uk/id/eprint/5280 |
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