Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy

McConnell, G. and Poland, S.P. and Girkin, J.M. (2006) Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy. Review of Scientific Instruments, 77 (013702). 013702. ISSN 0034-6748

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Official URL: http://dx.doi.org/10.1063/1.2148996

Abstract

We report on the use of a computer-controlled digital micromirror device (DMD) to select discrete wavelength ranges from a white-light supercontinuum and the application of the light source to confocal laser scanning microscopy (CLSM). The fast switching rate of the DMD enabled video-rate wavelength tuning, while the high resolution allowed precise control over the bandwidth selection. The DMD system was used in conjunction with a CLSM system to characterize the wavelength switching capability, prior to performing three-dimensional CLSM of a variety of multiple-labeled samples in order to demonstrate the efficacy and practical application of the system.

ORCID iDs

McConnell, G.

, Poland, S.P. and Girkin, J.M.;

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Item metadata

Item type:	Article
ID code:	5280
Dates:	Date Event 2006 Published
Keywords:	multiplexing, supercontinuum generation, micromirrors, fluorescence, optical microscopy, optical tuning, optical switches, photonics, biophotonics, Optics. Light, Instrumentation
Subjects:	Science > Physics > Optics. Light
Department:	Faculty of Science > Physics > Institute of Photonics Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user:	Strathprints Administrator
Date deposited:	01 Feb 2008
Last modified:	20 Jan 2021 17:29
URI:	https://strathprints.strath.ac.uk/id/eprint/5280

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