Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy

McConnell, G. and Poland, S.P. and Girkin, J.M. (2006) Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy. Review of Scientific Instruments, 77 (013702). 013702. ISSN 0034-6748

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Abstract

We report on the use of a computer-controlled digital micromirror device (DMD) to select discrete wavelength ranges from a white-light supercontinuum and the application of the light source to confocal laser scanning microscopy (CLSM). The fast switching rate of the DMD enabled video-rate wavelength tuning, while the high resolution allowed precise control over the bandwidth selection. The DMD system was used in conjunction with a CLSM system to characterize the wavelength switching capability, prior to performing three-dimensional CLSM of a variety of multiple-labeled samples in order to demonstrate the efficacy and practical application of the system.

Creators(s): McConnell, G. ORCID logoORCID: https://orcid.org/0000-0002-7213-0686, Poland, S.P. and Girkin, J.M.;
Item type:Article
ID code:5280
Keywords:multiplexing, supercontinuum generation, micromirrors, fluorescence, optical microscopy, optical tuning, optical switches, photonics, biophotonics, Optics. Light, Instrumentation
Subjects:Science > Physics > Optics. Light
Department:Faculty of Science > Physics > Institute of Photonics
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Strathprints Administrator
Date deposited:01 Feb 2008
Last modified:20 Jan 2021 17:29
URI:https://strathprints.strath.ac.uk/id/eprint/5280
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