A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots

Stewart, Alasdair J. and O'Reilly, Emmet J. and Moriarty, Roisin D. and Bertoncello, Paolo and Keyes, Tia E. and Forster, Robert J. and Dennany, Lynn (2015) A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots. Electrochimica Acta, 157. pp. 8-14. ISSN 0013-4686 (https://doi.org/10.1016/j.electacta.2015.01.073)

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Abstract

This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future.

ORCID iDs

Stewart, Alasdair J., O'Reilly, Emmet J., Moriarty, Roisin D., Bertoncello, Paolo, Keyes, Tia E., Forster, Robert J. and Dennany, Lynn ORCID logoORCID: https://orcid.org/0000-0002-5481-1066;
  • Item type:Article
    ID code:52297
    Dates:
    Date
    Event
    1 March 2015
    Published
    17 January 2015
    Published Online
    16 January 2015
    Accepted
    Notes:NOTICE: this is the author’s version of a work that was accepted for publication in Electrochimica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Electrochimica Acta, 157, (17/01/2015) http://dx.doi.org/10.1016/j.electacta.2015.01.073
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:31 Mar 2015 07:20
    Last modified:18 Nov 2024 02:54
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/52297
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