A time-resolved multifocal multiphoton microscope for high speed FRET imaging in vivo

Poland, Simon P. and Krstajic, Nikola and Coelho, Simao and Tyndall, David and Walker, Richard J. and Devauges, Viviane and Morton, Penny E. and Nicholas, Nicole S. and Richardson, Justin and Li, David Day-Ue and Suhling, Klaus and Wells, Claire M. and Parsons, Maddy and Henderson, Robert K. and Ameer-Beg, Simon M. (2014) A time-resolved multifocal multiphoton microscope for high speed FRET imaging in vivo. Optics Letters, 39 (20). pp. 6103-6106. ISSN 0146-9592 (https://doi.org/10.1364/OL.39.006013)

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Abstract

Imaging the spatio-temporal interaction of proteins in vivo is essential to understanding the complexities of biological systems. The highest accuracy monitoring of protein-protein interactions is achieved using FRET measured by fluorescence lifetime imaging with measurements taking minutes to acquire a single frame, limiting their use in dynamic live cell systems. We present a diffraction limited, massively parallel, time-resolved multifocal multiphoton microscope capable of producing fluorescence lifetime images with 55 ps time-resolution giving improvements in acquisition speed of a factor of 64. We present demonstrations with FRET imaging in a model cell system and demonstrate in vivo FLIM using a GTPase biosensor in the zebrafish embryo.

ORCID iDs

Poland, Simon P., Krstajic, Nikola, Coelho, Simao, Tyndall, David, Walker, Richard J., Devauges, Viviane, Morton, Penny E., Nicholas, Nicole S., Richardson, Justin, Li, David Day-Ue ORCID logoORCID: https://orcid.org/0000-0002-6401-4263, Suhling, Klaus, Wells, Claire M., Parsons, Maddy, Henderson, Robert K. and Ameer-Beg, Simon M.;
  • Item type:Article
    ID code:51464
    Dates:
    Date
    Event
    14 October 2014
    Published
    19 September 2014
    Accepted
    Notes:This paper was published in Optics Letters and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-20-601. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
    Subjects:Science > Physics > Optics. Light
    Medicine > Therapeutics. Pharmacology
    Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Depositing user: Pure Administrator
    Date deposited:05 Feb 2015 15:43
    Last modified:17 Nov 2024 01:09
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/51464
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