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Controlling cancer cell fate using localized biocatalytic self-assembly of an aromatic carbohydrate amphiphile

Pires, Ricardo A. and Abul-Haija, Yousef M. and Costa, Diana S. and Novoa-Carballal, Ramon and Reis, Rui L. and Ulijn, Rein V. and Pashkuleva, Iva (2014) Controlling cancer cell fate using localized biocatalytic self-assembly of an aromatic carbohydrate amphiphile. Journal of the American Chemical Society, 137 (2). pp. 576-579. ISSN 0002-7863

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Abstract

We report on a simple carbohydrate amphiphile able to self-assemble into nanofibers upon enzymatic dephosphorylation. The self-assembly can be triggered by alkaline phosphatase (ALP) in solution or in situ by the ALP produced by osteosarcoma cell line, SaOs2. In the latter case, assembly and localized gelation occurs mainly on the cell surface. The gelation of the pericellular environment induces a reduction of the SaOs2 metabolic activity at an initial stage (≥7 h) that results in cell death at longer exposure periods (≥24 h). We show that this effect depends on the phosphatase concentration, and thus, it is cell-selective with prechondrocytes ATDC5 (that express ∼15-20 times lower ALP activity compared to SaOs2) not being affected at concentrations ≥1 mM. These results demonstrate that simple carbohydrate derivatives can be used in an antiosteosarcoma strategy with limited impact on the surrounding healthy cells/tissues.

Item type: Article
ID code: 51329
Notes: This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in Journal of the American Chemical Society, copyright © American Chemical Society after peer review. To access the final edited and published work, see http://pubs.acs.org/doi/abs/10.1021/ja5111893
Keywords: cancer cell fate, cells, enzymatic dephosphorylation, localized gelation, self-assembly, biocatalysis, Chemistry, Biochemistry, Colloid and Surface Chemistry, Chemistry(all), Catalysis
Subjects: Science > Chemistry
Department: Faculty of Science > Pure and Applied Chemistry
Technology and Innovation Centre > Bionanotechnology
Faculty of Science
Depositing user: Pure Administrator
Date deposited: 28 Jan 2015 16:14
Last modified: 02 Dec 2018 13:52
Related URLs:
URI: https://strathprints.strath.ac.uk/id/eprint/51329
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