Fast bi-exponential fluorescence lifetime imaging analysis methods

Li, David Day-Uei and Yu, Hongqi and Chen, Yu (2015) Fast bi-exponential fluorescence lifetime imaging analysis methods. Optics Letters, 40 (3). pp. 336-339. ISSN 0146-9592 (

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A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are non-iterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They can be applicable to single-channel or 2D multi-channel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed non-fitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.