Plasmonic ELISA for the detection of gp120 at ultralow concentrations with the naked eye

Cecchin, D. and De La Rica, Roberto and Bain, R.E.S. and Finnis, Michael W. and Stevens, M.M. and Battaglia, G. (2014) Plasmonic ELISA for the detection of gp120 at ultralow concentrations with the naked eye. Nanoscale, 6 (16). pp. 9559-9562. ISSN 2040-3372

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Abstract

The technique of plasmonic ELISA is utilised here to detect the HIV-1 protein gp120 with the ultralow limit of detection of 8 × 10−20 M (10−17 g mL−1) in an independent laboratory. It was corroborated that changes in the concentration of hydrogen peroxide as small as 0.05 μM could lead to nanoparticle solutions of completely different tonality.