Improved protocols for vibrational spectroscopic analysis of body fluids
Bonnier, Franck and Petitjean, François and Baker, Matthew J. and Byrne, Hugh J. (2014) Improved protocols for vibrational spectroscopic analysis of body fluids. Journal of Biophotonics, 7 (3-4). pp. 167-179. ISSN 1864-0648 (https://doi.org/10.1002/jbio.201300130)
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The applications of vibrational spectroscopy to the examination of human blood serum are explored. Although FTIR spectra can be recorded in aqueous solutions at (gelatin) concentrations as low as 100 mg/L, the high-wavenumber region remains obscured by water absorption. Using Raman spectroscopy, high quality spectra of gelatine solutions as low as 10 mg/L can be achieved, also covering the high-wavenumber regions. In human serum, spectral profiles are weak and partially obscured by water features. Dried deposits are shown to be physically and chemically inhomogeneous resulting in reduced measurement reproducibility. Concentration of the serum using commercially available centrifugal filter devices results in an improvement in the spectral intensity and quality. Additionally, in Raman spectroscopy, reduced background and significantly enhanced signal collection is achievable by measurement in an inverted geometry. The improved protocols for spectroscopic measurement of human serum are applicable to a range of bodily fluids and should accelerate potential clinical applications.
ORCID iDs
Bonnier, Franck, Petitjean, François, Baker, Matthew J. ORCID: https://orcid.org/0000-0003-2362-8581 and Byrne, Hugh J.;-
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Item type: Article ID code: 50103 Dates: DateEvent1 April 2014Published17 October 2013Published Online23 September 2013AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 03 Nov 2014 14:09 Last modified: 10 Nov 2024 20:51 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/50103