Picture of DNA strand

Pioneering chemical biology & medicinal chemistry through Open Access research...

Strathprints makes available scholarly Open Access content by researchers in the Department of Pure & Applied Chemistry, based within the Faculty of Science.

Research here spans a wide range of topics from analytical chemistry to materials science, and from biological chemistry to theoretical chemistry. The specific work in chemical biology and medicinal chemistry, as an example, encompasses pioneering techniques in synthesis, bioinformatics, nucleic acid chemistry, amino acid chemistry, heterocyclic chemistry, biophysical chemistry and NMR spectroscopy.

Explore the Open Access research of the Department of Pure & Applied Chemistry. Or explore all of Strathclyde's Open Access research...

Metabolomic strategies in dereplication for targeted cultivation and isolation of new bioactive secondary metabolites from fungal endophytes and marine microbial symbionts

Cheng, Cheng and Abdelmohsen, Usama Ramada and Grkovic, Tanja and Quinn, Ronald and Hentschel, Ute and Edrada-Ebel, Ruangelie (2013) Metabolomic strategies in dereplication for targeted cultivation and isolation of new bioactive secondary metabolites from fungal endophytes and marine microbial symbionts. Planta Medica, 79 (13). ISSN 0032-0943

Full text not available in this repository.Request a copy from the Strathclyde author

Abstract

High resolution Fourier transform mass spectrometry (HRFTMS) and nuclear magnetic resonance (NMR) spectroscopy were employed as complimentary metabolomic tools to dereplicate chemical profiles of sponge-associated bacteria and fungal endophytes. The innovative strategy involved targeted cultivation and isolation of biologically active compounds. Principal Component (PCA), Hierarchical Clustering (HCA), and Orthogonal Partial Least Square-Discriminant (OPLS-DA) Analysis were used to analyze the HRFTMS and NMR data of culture extracts. The results of the statistical analysis identified and validated the best culture conditions and extraction procedure which optimized the isolation of novel bioactive metabolites. Sponge-derived actinomycetes have become a rich source of new natural products with interesting pharmacological activities. New O-glycosylated angucyclines, were isolated from the broth culture of Actinokineospora sp. strain EG49 which was cultivated from the Red Sea sponge Spheciospongia vagabunda. The structure of the compounds were determined by 1D- and 2D-NMR techniques as well as high resolution tandem mass spectrometry.