Metabolomic strategies in dereplication for targeted cultivation and isolation of new bioactive secondary metabolites from fungal endophytes and marine microbial symbionts

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Cheng, Cheng and Abdelmohsen, Usama Ramada and Grkovic, Tanja and Quinn, Ronald and Hentschel, Ute and Edrada-Ebel, Ruangelie (2013) Metabolomic strategies in dereplication for targeted cultivation and isolation of new bioactive secondary metabolites from fungal endophytes and marine microbial symbionts. Planta Medica, 79 (13). SL29. ISSN 0032-0943 (https://doi.org/10.1055/s-0033-1351855)

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Abstract

High resolution Fourier transform mass spectrometry (HRFTMS) and nuclear magnetic resonance (NMR) spectroscopy were employed as complimentary metabolomic tools to dereplicate chemical profiles of sponge-associated bacteria and fungal endophytes. The innovative strategy involved targeted cultivation and isolation of biologically active compounds. Principal Component (PCA), Hierarchical Clustering (HCA), and Orthogonal Partial Least Square-Discriminant (OPLS-DA) Analysis were used to analyze the HRFTMS and NMR data of culture extracts. The results of the statistical analysis identified and validated the best culture conditions and extraction procedure which optimized the isolation of novel bioactive metabolites. Sponge-derived actinomycetes have become a rich source of new natural products with interesting pharmacological activities. New O-glycosylated angucyclines, were isolated from the broth culture of Actinokineospora sp. strain EG49 which was cultivated from the Red Sea sponge Spheciospongia vagabunda. The structure of the compounds were determined by 1D- and 2D-NMR techniques as well as high resolution tandem mass spectrometry.

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