Surface display of the HPV L1 capsid protein by the autotransporter Shigella IcsA

Xu, Dan and Yang, Xiaofeng and Wang, Depu and Yu, Jun and Yili, Wang (2014) Surface display of the HPV L1 capsid protein by the autotransporter Shigella IcsA. Journal of Microbiology, 52 (1). pp. 77-82. ISSN 1976-3794

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Abstract

Autotransporters have become attractive tools for surface expression of foreign proteins in Gram-negative bacteria. In this study, the Shigella autotransporter IcsA, has been exploited to express the human papillomavirus (HPV) type 16 L1 capsid protein in Shigella sonnei and Escherichia coli. The L1 gene was fused in-frame to replace the coding sequence of the IcsA passenger domain that is responsible for actin-based motility. The resultant hybrid protein could be detected by an anti-L1 antibody on the surface of S. sonnei and E. coli. In E. coli, the protein was expressed on the entire surface of the bacterium. In contrast, the protein was detected mainly at one pole of the Shigella bacterium. However, the protein became evenly distributed on the surface of the Shigella bacterium when the icsP gene was removed. Our study demonstrated the possibility of exploiting autotransporters for surface expression of large, heterologous viral proteins, which may be a useful strategy for vaccine development.

Author(s):Xu, Dan, Yang, Xiaofeng, Wang, Depu, Yu, Jun and Yili, Wang
Item type:Article
ID code:49664
Keywords:autotransporter, gene fusion, surface expression, shigella sonnei, Pharmacy and materia medica, Virology, Medicine(all)
Subjects:Medicine > Pharmacy and materia medica
Science > Microbiology > Virology
Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Pure Administrator
Date deposited:07 Oct 2014 13:50
Last modified:11 Jan 2019 03:41
URI:https://strathprints.strath.ac.uk/id/eprint/49664
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