Picture map of Europe with pins indicating European capital cities

Open Access research with a European policy impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the European Policies Research Centre (EPRC).

EPRC is a leading institute in Europe for comparative research on public policy, with a particular focus on regional development policies. Spanning 30 European countries, EPRC research programmes have a strong emphasis on applied research and knowledge exchange, including the provision of policy advice to EU institutions and national and sub-national government authorities throughout Europe.

Explore research outputs by the European Policies Research Centre...

Deletion of IL-4Rα on CD4 T cells renders BALB/c mice resistant to Leishmania major infection

Radwanska, Magdalena and Cutler, Antony J. and Hoving, J. Claire and Magez, Stefan and Holscher, Christoph and Bohms, Andreas and Arendse, Berenice and Kirsch, Richard and Hunig, Thomas and Alexander, James and Kaye, Paul and Brombacher, Frank (2007) Deletion of IL-4Rα on CD4 T cells renders BALB/c mice resistant to Leishmania major infection. PLOS Pathogens, 3 (5). 0619-0629. ISSN 1553-7366

[img]
Preview
Other (Radwanska-etal-PLOSP-2007-Deletion-of-IL-4Rα-on-CD4-T-cells-renders-BALB-c-mice-resistant-to-Leishmania)
Radwanska_etal_PLOSP_2007_Deletion_of_IL_4R_on_CD4_T_cells_renders_BALB_c_mice_resistant_to_Leishmania.PDF - Final Published Version
License: Creative Commons Attribution 4.0 logo

Download (426kB) | Preview

Abstract

Effector responses induced by polarized CD4+T helper 2 (Th2) cells drive nonhealing responses in BALB/c mice infected with Leishmania major. Th2 cytokines IL-4 and IL-13 are known susceptibility factors for L. major infection in BALB/c mice and induce their biological functions through a common receptor, the IL-4 receptor α chain (IL-4Rα). IL-4Rα-deficient BALB/c mice, however, remain susceptible to L. major infection, indicating that IL-4/IL-13 may induce protective responses. Therefore, the roles of polarized Th2 CD4+T cells and IL-4/IL-13 responsiveness of non-CD4+T cells in inducing nonhealer or healer responses have yet to be elucidated. CD4+T cell-specific IL-4Rα (LckcreIL-4Rα/lox) deficient BALB/c mice were generated and characterized to elucidate the importance of IL-4Ra signaling during cutaneous leishmaniasis in the absence of IL-4-responsive CD4+T cells. Efficient deletion was confirmed by loss of IL-4Ra expression on CD4+T cells and impaired IL-4-induced CD4+T cell proliferation and Th2 differentiation. CD8+, γδ+, and NK-T cells expressed residual IL-4Ra, and representative non-T cell populations maintained IL-4/IL-13 responsiveness. In contrast to IL-4Rα/lox BALB/c mice, which developed ulcerating lesions following infection with L. major, LckcreIL-4Rα/lox mice were resistant and showed protection to rechallenge, similar to healer C57BL/6 mice. Resistance to L. major in LckcreIL-4Rα/lox mice correlated with reduced numbers of IL-10-secreting cells and early IL- 12p35 mRNA induction, leading to increased delayed type hypersensitivity responses, interferon-c production, and elevated ratios of inducible nitric oxide synthase mRNA/parasite, similar to C57BL/6 mice. These data demonstrate that abrogation of IL-4 signaling in CD4+T cells is required to transform nonhealer BALB/c mice to a healer phenotype. Furthermore, a beneficial role for IL-4Rα signaling in L. major infection is revealed in which IL-4/IL-13-responsive non-CD4+T cells induce protective responses.