A solid-state NMR study of the immobilization of alpha-chymotrypsin on mesoporous silica

Faure, N. E. and Halling, P. J. and Wimperis, S. (2014) A solid-state NMR study of the immobilization of alpha-chymotrypsin on mesoporous silica. Journal of Physical Chemistry C, 118 (2). pp. 1042-1048. (https://doi.org/10.1021/jp4098414)

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Abstract

Solid-state NMR spectroscopy was used to characterize a model biocatalyst system consisting of the enzyme α-chymotrypsin covalently immobilized on epoxide-silica ((glycidoxypropyl)trimethoxysilane, GOPS, grafted onto the surface of a silica gel). One- and two-dimensional 1H, 13C, and 29Si magic angle spinning (MAS) NMR techniques were employed. The support system (epoxide-silica) was characterized first and it was possible to assign silicon and carbon species in both the silica and the GOPS linker. After attachment of the protein, carbonyl carbons (175 ppm) in the immobilized enzyme were visible in 13C MAS NMR spectra recorded at B0 = 20 T. A number of further changes were observed in the 13C and 29Si MAS NMR spectra during the immobilization process, arising from a cross-linking of the surface silica species and an opening of the epoxide functional group by nucleophilic attack. This study shows the potential of multinuclear solid-state NMR for obtaining a better understanding of solid biocatalyst systems at the molecular level.

ORCID iDs

Faure, N. E., Halling, P. J. ORCID logoORCID: https://orcid.org/0000-0001-5077-4088 and Wimperis, S.;
  • Item type:Article
    ID code:48441
    Dates:
    Date
    Event
    16 January 2014
    Published
    20 December 2013
    Published Online
    Notes:This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Journal of Physical Chemistry C, copyright © American Chemical Society after peer review. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/jp4098414.
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:05 Jun 2014 12:59
    Last modified:11 Nov 2024 10:43
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/48441
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