Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Tools

Jeorrett, Abigail H and Neale, Steven L and Massoubre, David and Gu, Erdan and Henderson, Robert K and Millington, Owain and Mathieson, Keith and Dawson, Martin D (2014) Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells. Optics Express, 22 (2). pp. 1372-1380. ISSN 1094-4087 (https://doi.org/10.1364/OE.22.001372)

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Abstract

A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λmax 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.

ORCID iDs

Jeorrett, Abigail H, Neale, Steven L, Massoubre, David, Gu, Erdan ORCID logoORCID: https://orcid.org/0000-0002-7607-9902, Henderson, Robert K, Millington, Owain ORCID logoORCID: https://orcid.org/0000-0002-3026-6550, Mathieson, Keith ORCID logoORCID: https://orcid.org/0000-0002-9517-8076 and Dawson, Martin D ORCID logoORCID: https://orcid.org/0000-0002-6639-2989;
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