Hydrogels for the detection and management of protease levels
Patrick, Alison G. and Ulijn, Rein V. (2010) Hydrogels for the detection and management of protease levels. Macromolecular Bioscience, 10 (10). pp. 1184-1193. (https://doi.org/10.1002/mabi.200900457)
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The design of hydrogels that simultaneously report protease activity and remove excess protease from solution is elucidated. The hydrogels, based on amino-PEGA, combine enzyme-specific peptides flanked with FRET complimented by charged amino acid residues that facilitate protease uptake via short range electrostatic interactions. Enzymatic response was analysed using a combination of fluorescence spectroscopy, two-photon microscopy and UV/Vis spectroscopy. An optimised elastase-responsive hydrogel resulted in lowering of elastase levels below those typical of chronic wounds. The versatility of the modular-design approach was demonstrated by development of matrix metalloprotease and chymotrypsin sensitive systems.
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Item type: Article ID code: 46424 Dates: DateEvent8 October 2010Published30 June 2010Published OnlineSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 07 Jan 2014 12:12 Last modified: 04 May 2024 05:06 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/46424