Hydrogels for the detection and management of protease levels

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Patrick, Alison G. and Ulijn, Rein V. (2010) Hydrogels for the detection and management of protease levels. Macromolecular Bioscience, 10 (10). pp. 1184-1193. (https://doi.org/10.1002/mabi.200900457)

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Abstract

The design of hydrogels that simultaneously report protease activity and remove excess protease from solution is elucidated. The hydrogels, based on amino-PEGA, combine enzyme-specific peptides flanked with FRET complimented by charged amino acid residues that facilitate protease uptake via short range electrostatic interactions. Enzymatic response was analysed using a combination of fluorescence spectroscopy, two-photon microscopy and UV/Vis spectroscopy. An optimised elastase-responsive hydrogel resulted in lowering of elastase levels below those typical of chronic wounds. The versatility of the modular-design approach was demonstrated by development of matrix metalloprotease and chymotrypsin sensitive systems.

ORCID iDs

Patrick, Alison G. and Ulijn, Rein V. ORCID logoORCID: https://orcid.org/0000-0001-7974-3779;
CORE (COnnecting REpositories)