Plasmonic ELISA for the detection of analytes at ultralow concentrations with the naked eye

De La Rica, Roberto and Stevens, Molly M. (2013) Plasmonic ELISA for the detection of analytes at ultralow concentrations with the naked eye. Nature Protocols, 8. pp. 1759-1764. ISSN 1754-2189 (https://doi.org/10.1038/nprot.2013.085)

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Abstract

This protocol describes a signal-generation mechanism for the naked-eye detection of analytes at low concentrations with ELISA. The key step is to generate solutions of desired tonality by growing gold nanoparticles with a particular state of aggregation. This is accomplished by linking the growth of gold nanoparticles with the biocatalytic cycle of the enzyme label. The protocol adapts a conventional ELISA procedure with catalase-labeled antibodies. The enzyme consumes hydrogen peroxide, and then gold (III) ions are added to generate gold nanoparticles. The concentration of hydrogen peroxide dictates the state of aggregation of gold nanoparticles. This allows for the naked-eye detection of analytes by observing the generation of blue- or red-colored gold nanoparticle solutions. When coupled with conventional ELISA, this signal-generation procedure allows for the naked-eye detection of analytes within 1 h.

  • Item type:Article
    ID code:45647
    Dates:
    Date
    Event
    2013
    Published
    22 August 2013
    Published Online
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:05 Nov 2013 14:33
    Last modified:11 Nov 2024 10:32
    URI:https://strathprints.strath.ac.uk/id/eprint/45647
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