Plasmodium falciparum ATG8 implicated in both autophagy and apicoplast formation
Tomlins, Andrew M and Ben-Rached, Fathia and Williams, Roderick Am and Proto, William R and Coppens, Isabelle and Ruch, Ulrike and Gilberger, Tim W and Coombs, Graham H and Mottram, Jeremy C and Müller, Sylke and Langsley, Gordon (2013) Plasmodium falciparum ATG8 implicated in both autophagy and apicoplast formation. Autophagy, 9 (10). 1540 - 1552.
Full text not available in this repository.Request a copyAbstract
Amino acid utilization is important for the growth of the erythrocytic stages of the human malaria parasite Plasmodium falciparum, however the molecular mechanism that permits survival of the parasite during conditions of limiting amino acid supply is poorly understood. We provide data here suggesting that an autophagy pathway functions in P. falciparum despite the absence of a typical lysosome for digestion of the autophagosomes. It involves PfATG8, which has a C-terminal glycine which is absolutely required for association of the protein with autophagosomes. Amino acid starvation provoked increased colocalization between PfATG8- and PfRAB7-labeled vesicles and acidification of the colabeled structures consistent with PfRAB7-mediated maturation of PfATG8-positive autophagosomes; this is a rapid process facilitating parasite survival. Immuno-electron microscopic analyses detected PfRAB7 and PfATG8 on double-membrane-bound vesicles and also near or within the parasite's food vacuole, consistent with autophagosomes fusing with the endosomal system before being routed to the food vacuole for digestion. In nonstarved parasites, PfATG8, but not PfRAB7, was found on the intact apicoplast membrane and on apicoplast-targeted vesicles and apicoplast remnants when the formation of the organelle was disrupted; a localization also requiring the C-terminal glycine. These findings suggest that in addition to a classical role in autophagy, which involves the PfRAB7-endosomal system and food vacuole, PfATG8 is associated with apicoplast-targeted vesicles and the mature apicoplast, and as such contributes to apicoplast formation and maintenance. Thus, PfATG8 may be unique in having such a second role in addition to the formation of autophagosomes required for classical autophagy.
-
-
Item type: Article ID code: 45131 Dates: DateEvent29 August 2013PublishedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 14 Oct 2013 10:52 Last modified: 08 Sep 2024 01:44 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/45131