Two-photon laser scanning fluorescence microscopy using photonic crystal fibre

McConnell, G. and Riis, E. (2004) Two-photon laser scanning fluorescence microscopy using photonic crystal fibre. Journal of Biomedical Optics, 9 (5). pp. 922-927. ISSN 1083-3668 (https://doi.org/10.1117/1.1778734)

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Abstract

We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques. This system provided more than a 7-fold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of infrared radiaton of less than 35 fs duration reaching the sample.

ORCID iDs

McConnell, G. ORCID: https://orcid.org/0000-0002-7213-0686

Riis, E. ORCID: https://orcid.org/0000-0002-3225-5302

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• Item metadata

  Item type:	Article
  ID code:	45
  Dates:	Date Event 2004 Published
  Subjects:	Science > Physics > Optics. Light
  Department:	Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Faculty of Science > Physics
  Depositing user:	Miss Jacqueline Miller
  Date deposited:	14 Dec 2005
  Last modified:	11 Nov 2024 08:26
  URI:	https://strathprints.strath.ac.uk/id/eprint/45