Lethal effects of high intensity violet 405-nm light on saccharomyces cerevisiae, candida albicans and on dormant and germinating spores of aspergillus niger

Murdoch, L.E. and McKenzie, K. and MacLean, M. and MacGregor, S.J. and Anderson, J.G. (2013) Lethal effects of high intensity violet 405-nm light on saccharomyces cerevisiae, candida albicans and on dormant and germinating spores of aspergillus niger. Fungal biology, 117 (7-8). pp. 519-527. ISSN 1878-6146 (https://doi.org/10.1016/j.funbio.2013.05.004)

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Abstract

This study assessed the effects of high-intensity violet light on selected yeast and mould fungi. Cell suspensions of Saccharomyces cerevisiae, Candida albicans, and dormant and germinating spores (conidia) of the mould Aspergillus niger were exposed to high-intensity narrow band violet light with peak output at 405 nm generated from a light-emitting diode (LED) array. All three fungal species were inactivated by the 405-nm light without a requirement for addition of exogenous photosensitiser chemicals. Of the fungal species tested, S. cerevisiae was most sensitive and dormant conidia of A. niger were most resistant to 405-nm light exposure. Five-log10 colony forming units per millilitre (CFU ml1) reductions of the tested species required exposure doses of 288 J cm2 for S. cerevisiae, 576 J cm2 for C. albicans, and a much higher value of 2.3 kJ cm2 for dormant conidia of A. niger. During germination, A. niger conidia became more sensitive to 405-nm light exposure and sensitivity increased as germination progressed over an 8 h test period. Light exposure under aerobic and anaerobic conditions, together with results obtained using ascorbic acid as a scavenger of reactive oxygen species, revealed that 405-nm light inactivation in fungi involved an oxygen-dependent mechanism, as previously described in bacteria. The inactivation results achieved with yeast cells and fungal spores together with operational advantages associated with the use of a visible (nonultraviolet (UV)) light source highlight the potential of 405-nm light for fungal decontamination applications.

ORCID iDs

Murdoch, L.E., McKenzie, K., MacLean, M. ORCID logoORCID: https://orcid.org/0000-0001-5750-0397, MacGregor, S.J. ORCID logoORCID: https://orcid.org/0000-0002-0808-585X and Anderson, J.G. ORCID logoORCID: https://orcid.org/0000-0003-4151-1619;
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