Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein

Pickup, John and Khan, Faaizah and Zhi, Zheng-Liang and Coulter, Jonathan and Birch, David J S (2013) Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein. Journal of Diabetes Science and Technology, 7 (1). pp. 62-71. ISSN 1932-2968

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Abstract

We review recent progress in our laboratories towards developing in vivo glucose sensors for diabetes that are based on fluorescence labelling of glucose/galactose-binding protein. Measurement strategies have included both monitoring glucose-induced changes in fluorescence resonance energy transfer and labelling with the environmentally sensitive fluorophore, badan. Measuring fluorescence lifetime rather than intensity has particular potential advantages for in vivo sensing. A prototype fiber optic-based glucose sensor using this technology is being tested.