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Open Access research which pushes advances in bionanotechnology

Strathprints makes available scholarly Open Access content by researchers in the Strathclyde Institute of Pharmacy & Biomedical Sciences (SIPBS) , based within the Faculty of Science.

SIPBS is a major research centre in Scotland focusing on 'new medicines', 'better medicines' and 'better use of medicines'. This includes the exploration of nanoparticles and nanomedicines within the wider research agenda of bionanotechnology, in which the tools of nanotechnology are applied to solve biological problems. At SIPBS multidisciplinary approaches are also pursued to improve bioscience understanding of novel therapeutic targets with the aim of developing therapeutic interventions and the investigation, development and manufacture of drug substances and products.

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Designing peptide actuators for enzyme-responsive particles

Ulijn, Rein V. and Mart, Robert J. and McDonald, Thomas T. and Thornton, Paul D. (2009) Designing peptide actuators for enzyme-responsive particles. Abstracts of papers - American Chemical Society, 238. ISSN 0065-7727

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Abstract

We will report on enzyme-responsive hydrogel particles for capture and controlled release applications. These particles are functionalised with peptide actuators that simultaneously act as biorecognition elements and molecular actuators, triggering swelling or collapse of particles in response to enzymes under constant, physiological conditions. Amino-functionalised poly(ethylene glycol acrylamide) (PEGA) hydrogel particles were functionalised with peptide actuators using solid-phase synthesis approach directly on the polymer material. We demonstrate that these enzyme responsive particles can be designed to (i) achieve an increase or decrease of molecular accessibility of the polymer, as required; (ii) match the specificity of the target enzyme; (iii) match the size and charge properties of the to-be released protein payload, thereby uniquely allowing for tuneable release profiles and (iv) match the ionic strength of the environment. Release profiles were analysed using a combination of fluorescence spectroscopy of the solution and two-photon fluorescence microscopy to analyse enzymatically triggered molecular events within hydrogel particles during the initial stages of release.