POLY 385-interfacing LCDs and bioresponsive hydrogels
Birchall, Louise S. and Ulijn, Rein V. and Webb, Simon J. (2009) POLY 385-interfacing LCDs and bioresponsive hydrogels. Abstracts of papers - American Chemical Society, 238. ISSN 0065-7727
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We report the use of enzyme-responsive peptide hydrogels interfaced with LCD detection to give robust, power-free, portable and inexpensive sensing of proteases. In this approach hydrogel building blocks are contained within a sensor chamber. Addition of a protease results in cleavage of the hydrogel components producing Fmoc-peptide fragments which no longer self-assemble. Gel to solution transitions trigger an LCD response which can be visualised by placing the chamber between crossed polarisers: bright to dark transitions in the LC brightness indicate a positive optical response. Presented are our results for three systems where enzymatic hydrolysis of hydrogels could be followed by optical microscopy. A decrease in transmission was quantified via pixel counting and extent of hydrolysis followed by HPLC. Gel to solution transitions were monitored independently by fluorescence spectroscopy and imaged by AFM
Creators(s): |
Birchall, Louise S., Ulijn, Rein V. ![]() | Item type: | Article |
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ID code: | 43938 |
Keywords: | interfacing LCDs, bioresponsive hydrogels, enzyme-responsive peptide hydrogels, LCD detection, Chemistry, Chemistry(all) |
Subjects: | Science > Chemistry |
Department: | Faculty of Science > Pure and Applied Chemistry |
Depositing user: | Pure Administrator |
Date deposited: | 30 May 2013 11:17 |
Last modified: | 17 Dec 2020 01:57 |
URI: | https://strathprints.strath.ac.uk/id/eprint/43938 |
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