Sequence-selective detection of double-stranded DNA sequences using pyrrole-imidazole polyamide microarrays

Singh, Ishwar and Wendeln, Christian and Clark, Alasdair W. and Cooper, Jonathan M. and Ravoo, Bart Jan and Burley, Glenn A. (2013) Sequence-selective detection of double-stranded DNA sequences using pyrrole-imidazole polyamide microarrays. Journal of the American Chemical Society, 135 (9). pp. 3449-3457. ISSN 1520-5126 (https://doi.org/10.1021/ja309677h)

Preview

Text. Filename: Singh_etal_JACS2013_Sequence_selective_detection_of_double_stranded_DNA.pdf Final Published Version License: Download (8MB)| Preview

Abstract

We describe a microarray format that can detect double-stranded DNA sequences with a high degree of sequence selectivity. Cyclooctyne-derivatized pyrrole-imidazole polyamides were immobilized on azide-modified glass substrates using microcontact printing and a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. These polyamide-immobilized substrates selectively detected a seven-base-pair binding site incorporated within a double-stranded oligodeoxyribonucleotide sequence even in the presence of an excess of a sequence with a single-base-pair mismatch

• Share and Export
  

  RefWorksRIS (EndNote Online, Zotero, Ref manager, etc)RDF+XMLEndNoteASCII CitationOpenURL ContextObject in SpanMPEG-21 DIDLRIOXX2 XMLBibTeXAtomEP3 XMLDublin CoreRDF+N-TriplesMODSHTML CitationJSONData Cite XMLRDF+N3Multiline CSVOpenURL ContextObjectReferSimple MetadataMETS
• Item metadata

  Item type:	Article
  ID code:	43295
  Dates:	Date Event 6 March 2013 Published 21 February 2013 Published Online
  Subjects:	Science > Chemistry
  Department:	Faculty of Science > Pure and Applied Chemistry Technology and Innovation Centre > Bionanotechnology
  Depositing user:	Pure Administrator
  Date deposited:	21 Mar 2013 12:01
  Last modified:	11 Nov 2024 10:22
  Related URLs:	Journal or Publication
  URI:	https://strathprints.strath.ac.uk/id/eprint/43295