Sequence-selective detection of double-stranded DNA sequences using pyrrole-imidazole polyamide microarrays

Singh, Ishwar and Wendeln, Christian and Clark, Alasdair W. and Cooper, Jonathan M. and Ravoo, Bart Jan and Burley, Glenn A. (2013) Sequence-selective detection of double-stranded DNA sequences using pyrrole-imidazole polyamide microarrays. Journal of the American Chemical Society, 135 (9). pp. 3449-3457. ISSN 1520-5126 (https://doi.org/10.1021/ja309677h)

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Abstract

We describe a microarray format that can detect double-stranded DNA sequences with a high degree of sequence selectivity. Cyclooctyne-derivatized pyrrole-imidazole polyamides were immobilized on azide-modified glass substrates using microcontact printing and a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. These polyamide-immobilized substrates selectively detected a seven-base-pair binding site incorporated within a double-stranded oligodeoxyribonucleotide sequence even in the presence of an excess of a sequence with a single-base-pair mismatch

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