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Open Access research with a European policy impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the European Policies Research Centre (EPRC).

EPRC is a leading institute in Europe for comparative research on public policy, with a particular focus on regional development policies. Spanning 30 European countries, EPRC research programmes have a strong emphasis on applied research and knowledge exchange, including the provision of policy advice to EU institutions and national and sub-national government authorities throughout Europe.

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Sporicidal effects of high-intensity 405 nm visible light on endospore-forming bacteria

MacLean, Michelle and Murdoch, Lynne and MacGregor, Scott and Anderson, John (2013) Sporicidal effects of high-intensity 405 nm visible light on endospore-forming bacteria. Photochemistry and Photobiology, 89 (1). pp. 120-126. ISSN 0031-8655

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Abstract

Resistance of bacterial endospores to treatments including biocides, heat and radiation is a persistent problem. This study investigates the susceptibility of Bacillus and Clostridium endospores to 405-nm visible light, wavelengths which have been shown to induce inactivation of vegetative bacterial cells. Suspensions of B. cereus endospores were exposed to high-intensity 405-nm light generated from a light-emitting diode array and results demonstrate the induction of a sporicidal effect. Up to a 4-log10 CFU mL-1 reduction in spore population was achieved after exposure to a dose of 1.73 kJcm-2. Similar inactivation kinetics were demonstrated with B. subtilis, B. megaterium and C. difficile endospores. The doses required for inactivation of endospores were significantly higher than those required for inactivation of B. cereus and C. difficile vegetative cells, where approximately 4-log10 CFU mL-1 reductions were achieved after exposure to doses of 108 and 48 Jcm-2, respectively. The significant increase in dose required for inactivation of endospores compared to vegetative cells is unsurprising due to the notorious resilience of these microbial structures. However, the demonstration that visible light of 405-nm can induce a bactericidal effect against endospores is significant, and could have potential for incorporation into decontamination methods for the removal of bacterial contamination including endospores.