Novel disposable biochip platform employing supercritical angle fluorescence for enhanced fluorescence collection
Hill, D. and McDonnell, B. and Hearty, S. and Basabe-Desmonts, L. and Blue, R. and Trnavsky, M. and McAtamney, C. and O’Kennedy, R. and MacCraith, B. D. (2011) Novel disposable biochip platform employing supercritical angle fluorescence for enhanced fluorescence collection. Biomedical Microdevices, 13 (4). pp. 759-767. (https://doi.org/10.1007/s10544-011-9546-2)
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This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a planar substrate. The optical elements are based on supercritical angle fluorescence (SAF) which provides substantial enhancement of the fluorescence collection efficiency but also confines the fluorescence detection volume strictly to the immediate proximity of the biochip surface, thereby having the potential to discriminate against background fluorescence from the analyte solution. An optical reader is also described that enables interrogation and fluorescence collection from the nine optical elements on the chip. The sensitivity of the system was determined with a biotin-avidin assay while its clinical utility was demonstrated in an assay for C-reactive protein (CRP), an inflammation marker.
ORCID iDs
Hill, D., McDonnell, B., Hearty, S., Basabe-Desmonts, L., Blue, R. ORCID: https://orcid.org/0000-0002-8598-5210, Trnavsky, M., McAtamney, C., O’Kennedy, R. and MacCraith, B. D.;-
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Item type: Article ID code: 42842 Dates: DateEventAugust 2011PublishedSubjects: Technology > Electrical engineering. Electronics Nuclear engineering Department: Faculty of Engineering > Electronic and Electrical Engineering Depositing user: Pure Administrator Date deposited: 12 Feb 2013 20:45 Last modified: 11 Nov 2024 10:20 URI: https://strathprints.strath.ac.uk/id/eprint/42842