Synthesis of vesicle cargo determines amplitude of Ca2+-sensitive exocytosis

Sutter, Jens and Denecke, Juergen and Thiel, Gerhard (2012) Synthesis of vesicle cargo determines amplitude of Ca2+-sensitive exocytosis. Cell Calcium, 52 (3-4). pp. 283-288. ISSN 0143-4160 (https://doi.org/10.1016/j.ceca.2012.05.011)

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Abstract

Here we examine the potential coupling between the synthesis of secretory proteins and the sensitivity of exocytosis to the concentration of free Ca2+ in the cytosol ([Ca2+](i)) in plant cell. We therefore monitor in tobacco protoplasts the excursion of the membrane capacitance in response to an elevation of[Ca2+](i) as a measure for exocytotic activity. The data show that a ramp like elevation of [Ca2+](i) generates in protoplasts from wild type plants and from transgenic plants, which overexpress the secreted alpha-amylase, an exocytotic burst with an initial steep and a subsequent slow phase. The largest capacitive burst is obtained in alpha-amylase producing plants and the amplitude of the [Ca2+](i) evoked C-m excursion is a function of the amylase synthesis of the plants. The data support a model according to which plant cells have at least two serial [Ca2+](i) sensitive processes in the final steps of their exocytotic pathway. The overproduction of a secreted cargo does not affect the kinetics of this process but the number of vesicles in pools upstream of the [Ca2+](i) sensitive steps. (C) 2012 Elsevier Ltd. All rights reserved.

ORCID iDs

Sutter, Jens ORCID logoORCID: https://orcid.org/0000-0002-3782-6338, Denecke, Juergen and Thiel, Gerhard;
  • Item type:Article
    ID code:42286
    Dates:
    Date
    Event
    2012
    Published
    8 June 2012
    Published Online
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:05 Dec 2012 14:36
    Last modified:11 Nov 2024 10:17
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/42286
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