Fluorescence anisotropy metrology of electrostatically and covalently labelled silica nanoparticles
Yip, Philip and Karolin, Jan and Birch, David J. S. (2012) Fluorescence anisotropy metrology of electrostatically and covalently labelled silica nanoparticles. Measurement Science and Technology, 23 (8). -. 084003. ISSN 0957-0233 (https://doi.org/10.1088/0957-0233/23/8/084003)
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We compare determining the size of silica nanoparticles using the time-resolved fluorescence anisotropy decay of dye molecules when electrostatically and covalently bound to stable silica nanoparticles. Covalent labelling is shown to offer advantages by simplifying the dye rotational kinetics and the appropriateness of various kinetic models is discussed. Silica nanoparticles produced using Stober synthesis of tetraethylorthosilicate (TEOS) are found to be controllable between similar to 3.1 and 3.8 nm radius by adjusting the relative water: TEOS concentration. Covalent labelling with fluorescein 5(6)-isothiocyanate (FITC) bound to (3-aminopropyl) trimethoxysilane (FITC-APS) predicts a larger particle than electrostatically labelling with rhodamine 6G. The difference is attributed to the presence of an additional depolarization mechanism to Brownian rotation of the nanoparticle and dye wobbling with electrostatic labelling in the form of dye diffusion on the surface of the nanoparticle.
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Item type: Article ID code: 41440 Dates: DateEventAugust 2012PublishedSubjects: Science > Physics > Solid state physics. Nanoscience Department: Faculty of Science > Physics
Technology and Innovation Centre > BionanotechnologyDepositing user: Pure Administrator Date deposited: 15 Oct 2012 10:00 Last modified: 25 Apr 2024 17:14 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/41440