Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I

Thanakiatkrai, Phuvadol and Welch, Lindsey (2012) Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I. International Journal of Legal Medicine, 126 (1). pp. 161-165. ISSN 0937-9827 (https://doi.org/10.1007/s00414-011-0558-5)

Abstract

Here, we applied the Taguchi method, an engineering optimization process, to successfully determine the optimal conditions for three SYBR Green I-based quantitative PCR assays. This method balanced the effects of all factors and their associated levels by using an orthogonal array rather than a factorial array. Instead of running 27 experiments with the conventional factorial method, the Taguchi method achieved the same optimal conditions using only nine experiments, saving valuable resources.

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• Item metadata

  Item type:	Article
  ID code:	40204
  Dates:	Date Event 2012 Published
  Subjects:	Science > Chemistry
  Department:	Faculty of Science > Pure and Applied Chemistry
  Depositing user:	Pure Administrator
  Date deposited:	22 Jun 2012 15:36
  Last modified:	11 Nov 2024 10:09
  Related URLs:	Scopus publication
  URI:	https://strathprints.strath.ac.uk/id/eprint/40204