Identification of nucleic acid sequences

Faulds, Karen (2009) Identification of nucleic acid sequences. C12Q 1/68 (2006.1).

Abstract

The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.

ORCID iDs

Faulds, Karen ORCID: https://orcid.org/0000-0002-5567-7399

Graham, Duncan ORCID: https://orcid.org/0000-0002-6079-2105

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• Item metadata

  Item type:	Patent
  ID code:	36764
  Dates:	Date Event 19 February 2009 Published
  Keywords:	Raman spectroscopy , nucleic acid sequences, spectroscopy , Chemistry
  Subjects:	Science > Chemistry
  Department:	Faculty of Science > Pure and Applied Chemistry
  Depositing user:	Pure Administrator
  Date deposited:	17 Jan 2012 10:11
  Last modified:	18 Jan 2023 12:05
  URI:	https://strathprints.strath.ac.uk/id/eprint/36764