Picture of DNA strand

Pioneering chemical biology & medicinal chemistry through Open Access research...

Strathprints makes available scholarly Open Access content by researchers in the Department of Pure & Applied Chemistry, based within the Faculty of Science.

Research here spans a wide range of topics from analytical chemistry to materials science, and from biological chemistry to theoretical chemistry. The specific work in chemical biology and medicinal chemistry, as an example, encompasses pioneering techniques in synthesis, bioinformatics, nucleic acid chemistry, amino acid chemistry, heterocyclic chemistry, biophysical chemistry and NMR spectroscopy.

Explore the Open Access research of the Department of Pure & Applied Chemistry. Or explore all of Strathclyde's Open Access research...

Strathprints

Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks

Jefferies, Johanna M C and Johnston, Calum H G and Kirkham, Lea-Ann S and Cowan, Graeme J M and Ross, Kirsty S and Smith, Andrew and Clarke, Stuart C and Brueggemann, Angela B and George, Robert C and Pichon, Bruno and Pluschke, Gerd and Pfluger, Valentin and Mitchell, Tim J (2007) Presence of nonhemolytic pneumolysin in serotypes of Streptococcus pneumoniae associated with disease outbreaks. Journal of Infectious Diseases, 196 (6). pp. 936-944.

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

Pneumolysin is an important virulence factor of the human pathogen Streptococcus pneumoniae. Sequence analysis of the ply gene from 121 clinical isolates of S. pneumoniae uncovered a number of alleles. Twenty-two strains were chosen for further analysis, and 14 protein alleles were discovered. Five of these had been reported previously, and the remaining 9 were novel. Cell lysates were used to determine the specific hemolytic activities of the pneumolysin proteins. Six strains showed no hemolytic activity, and the remaining 16 were hemolytic, to varying degrees. We report that the nonhemolytic allele reported previously in serotype 1, sequence type (ST) 306 isolates is also present in a number of pneumococcal isolates of serotype 8 that belong to the ST53 lineage. Serotype 1 and 8 pneumococci are known to be associated with outbreaks of invasive disease. The nonhemolytic pneumolysin allele is therefore associated with the dominant clones of outbreak-associated serotypes of S. pneumoniae.

Item type: Article
ID code: 35749
Keywords: alleles, bacterial proteins, cluster analysis, DNA, bacterial, disease outbreaks, genotype, hemolysis, humans, molecular sequence data, pneumococcal infections, polymorphism, genetic, sequence analysis, DNA, sequence homology, serotyping, streptococcus pneumoniae, streptolysins, virulence factors, Pharmacy and materia medica, Infectious Diseases, Immunology and Allergy
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Pure Administrator
Date deposited: 11 Nov 2011 16:21
Last modified: 26 Apr 2019 02:18
URI: https://strathprints.strath.ac.uk/id/eprint/35749
Export data:
CORE (COnnecting REpositories)