Clustered integrin α5β1 ligand displays model fibronectin-mediated adhesion of human endometrial stromal cells
Li, Zhaohui and Kreiner, Michaela and van der Walle, Christopher F and Mardon, Helen J (2011) Clustered integrin α5β1 ligand displays model fibronectin-mediated adhesion of human endometrial stromal cells. Biochemical and Biophysical Research Communications, 407 (4). pp. 777-782. (https://doi.org/10.1016/j.bbrc.2011.03.099)
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Progress towards endometrial tissue engineering for modelling endometrial diseases and infertility is frustrated by the inability to mimic the fibronectin (FN) extracellular matrix required by human endometrial stromal cells (EnSCs). Here we show that this is because of the requirement to present integrin α5β1 (the FN receptor) ligands in specifically oriented, polyvalent displays; by engineering controlled self-assembly of the 9th-10th type III FN domain pair (FIII9-10, the minimal integrin α5β1 ligand) immobilised in a specific orientation to cell culture surfaces. The fraction of adherent EnSCs seen to spread increased significantly for the multimeric ligand surfaces in the order: tetramer>trimer>dimer>monomer. The extent of EnSC spread morphology also increased in the same order, with the tetrameric ligand supporting a morphology most similar to that supported by FN. Our data suggest that only higher-order multimers of FIII9-10 will fully promote cell spreading mediated through integrin α5β1 binding.
ORCID iDs
Li, Zhaohui, Kreiner, Michaela ORCID: https://orcid.org/0000-0003-4824-0153, van der Walle, Christopher F and Mardon, Helen J;-
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Item type: Article ID code: 35154 Dates: DateEvent22 April 2011PublishedNotes: Copyright © 2011 Elsevier Inc. All rights reserved. Subjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 25 Oct 2011 15:46 Last modified: 11 Nov 2024 09:56 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/35154