Impaired Gluconeogenesis in a porcine model of paracetamol induced acute liver failure

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Dabos, Konstantinos and Whalen, Henry and Newsome, Philip and Parkinson, John and Henderson, Neil and Sadler, Ian and Hayes, Peter and Plevris, John (2011) Impaired Gluconeogenesis in a porcine model of paracetamol induced acute liver failure. World Journal of Gastroenterology, 17 (11). pp. 1457-1461. ISSN 1007-9327 (https://doi.org/10.3748/wjg.v17.i11.1457)

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Abstract

To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF). Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using (1)H NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples. No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05). Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state.

ORCID iDs

Dabos, Konstantinos, Whalen, Henry, Newsome, Philip, Parkinson, John ORCID logoORCID: https://orcid.org/0000-0003-4270-6135, Henderson, Neil, Sadler, Ian, Hayes, Peter and Plevris, John;
  • Item type:Article
    ID code:34141
    Dates:
    Date
    Event
    21 March 2011
    Published
    Subjects:Science
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:20 Oct 2011 09:25
    Last modified:11 Nov 2024 09:52
    URI:https://strathprints.strath.ac.uk/id/eprint/34141
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