Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+)

Dennany, L and Forster, R J and White, B and Smyth, M and Rusling, J F (2004) Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)(2)(PVP)(10)](2+). Journal of the American Chemical Society, 126 (28). pp. 8835-8841. ISSN 0002-7863 (https://doi.org/10.1021/ja048615+)

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Abstract

Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.

ORCID iDs

Dennany, L ORCID logoORCID: https://orcid.org/0000-0002-5481-1066, Forster, R J, White, B, Smyth, M and Rusling, J F;
  • Item type:Article
    ID code:34098
    Dates:
    Date
    Event
    21 July 2004
    Published
    Keywords:mediated fenton reactions, hydrogen-peroxide, oxidative stress, oxygen radicals, electrochemical oxidation, strand breaks, in-vivo, iron, Chemistry, Biochemistry, Colloid and Surface Chemistry, Chemistry(all), Catalysis
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:20 Oct 2011 12:46
    Last modified:06 Aug 2021 02:41
    URI:https://strathprints.strath.ac.uk/id/eprint/34098
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