Picture of rolled up £5 note

Open Access research that shapes economic thinking...

Strathprints makes available scholarly Open Access content by the Fraser of Allander Institute (FAI), a leading independent economic research unit focused on the Scottish economy and based within the Department of Economics. The FAI focuses on research exploring economics and its role within sustainable growth policy, fiscal analysis, energy and climate change, labour market trends, inclusive growth and wellbeing.

The open content by FAI made available by Strathprints also includes an archive of over 40 years of papers and commentaries published in the Fraser of Allander Economic Commentary, formerly known as the Quarterly Economic Commentary. Founded in 1975, "the Commentary" is the leading publication on the Scottish economy and offers authoritative and independent analysis of the key issues of the day.

Explore Open Access research by FAI or the Department of Economics - or read papers from the Commentary archive [1975-2006] and [2007-2018]. Or explore all of Strathclyde's Open Access research...

A method to identify a large number of mammalian species in the UK from trace samples and mixtures without the use of sequencing

Tobe, Shanan S. and Linacre, Adrian (2008) A method to identify a large number of mammalian species in the UK from trace samples and mixtures without the use of sequencing. Forensic Science International: Genetics Supplement Series, 1 (1). pp. 625-627. ISSN 1875-1768

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

There is no standard test to identify the species of origin of a sample. A general method is to amplify part of the mitochondrial genome, generally the 12S, 16S or cytochrome b gene, and sequence it for comparison with known sequences on GenBank. Highly degraded samples and mixtures make this technique unsuitable. As a functioning protein, cytochrome b cannot mutate unconditionally. Detrimental changes in the amino acid sequence or composition will result in cell death and would not be passed on to offspring. By examining the cytochrome b sequences non-detrimental variation can be found which can be used for specific-species identification. Areas of high homology can also be identified for universal amplification sites. Species-specific primers have been developed based on these SNPs in the cytochrome b gene such that they will only react for a particular species. By combining universal priming sites with species-specific sites, a simple yet effect test has been constructed for the identification of species. This test will produce a product of a particular size for each species. It will work on mixtures and has sensitivity to the femtogramme (10−15 g) level.