Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose

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Rolinski, O.J. and Birch, D.J.S. and McCartney, L. and Pickup, J.C. (2001) Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 57 (11). pp. 2245-2254. ISSN 1386-1425 (https://doi.org/10.1016/S1386-1425(01)00475-9)

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Abstract

A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Förster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.

ORCID iDs

Rolinski, O.J. ORCID logoORCID: https://orcid.org/0000-0002-7838-779X, Birch, D.J.S. ORCID logoORCID: https://orcid.org/0000-0001-6400-1270, McCartney, L. and Pickup, J.C.;
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