Fluorescence nanotomography using resonance energy transfer: Demonstration with a protein-sugar complex

Rolinski, Olaf J. and Birch, David J.S. and McCartney, Lydia J. and Pickup, John C. (2001) Fluorescence nanotomography using resonance energy transfer: Demonstration with a protein-sugar complex. Physics in Medicine and Biology, 46 (2001). N221-N226. ISSN 0031-9155

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Abstract

A new approach to structural sensing, fluorescence resonance energy transfer nanotomography, which interprets fluorescence decay measurement in terms of site density analysis of molecular distributions, has been applied to a glucose sensor based on competitive binding with malachite green labelled dextran to the sugar binding protein concanavalinA labelled with allophycocyanin. Opportunities for structural sensing in clinical medicine are highlighted.

ORCID iDs

Rolinski, Olaf J. ORCID logoORCID: https://orcid.org/0000-0002-7838-779X, Birch, David J.S. ORCID logoORCID: https://orcid.org/0000-0001-6400-1270, McCartney, Lydia J. and Pickup, John C.;