A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein
Saxl, Tania and Khan, Faaizah and Ferla, Matteo and Birch, David and Pickup, John (2011) A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein. Analyst, 136 (5). pp. 968-972. ISSN 0003-2654 (https://doi.org/10.1039/c0an00430h)
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Abstract
Alternative, non-electrochemistry-based technologies for continuous glucose monitoring are needed for eventual use in diabetes mellitus. As part of a programme investigating fluorescent glucose sensors, we have developed fibre-optic biosensors using glucose/galactose binding protein (GBP) labelled with the environmentally sensitive fluorophore, Badan. GBP-Badan was attached via an oligohistidine-tag to the surface of Ni-nitrilotriacetic acid (NTA)-functionalized agarose or polystyrene beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the GBP-Badan-beads. Either GBP-Badan agarose or polystyrene beads were loaded into a porous chamber at the end of a multimode optical fibre. Fluorescence lifetime responses were recorded using pulsed laser excitation, high speed photodiode detection and time-correlated single-photon counting. The maximal response was at 100 mM glucose with an apparent K-d of 13 mM (agarose) and 20 mM (polystyrene), and good working-day stability was demonstrated. We conclude that fluorescence lifetime fibre-optic glucose sensors based on GBP-Badan are suitable for development as clinical glucose monitors.
ORCID iDs
Saxl, Tania, Khan, Faaizah, Ferla, Matteo, Birch, David ORCID: https://orcid.org/0000-0001-6400-1270 and Pickup, John;-
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Item type: Article ID code: 30189 Dates: DateEvent2011Published17 December 2010Published OnlineSubjects: Science > Chemistry > Physical and theoretical chemistry Department: Faculty of Science > Physics
Technology and Innovation Centre > BionanotechnologyDepositing user: Pure Administrator Date deposited: 30 Mar 2011 09:51 Last modified: 11 Nov 2024 09:42 URI: https://strathprints.strath.ac.uk/id/eprint/30189