Understanding enzyme action on immobilised substrates

Halling, P.J. and Ulijn, R.V. and Flitsch, S.L. (2005) Understanding enzyme action on immobilised substrates. Current Opinion in Biotechnology, 16 (4). pp. 385-392. ISSN 0958-1669 (http://dx.doi.org/10.1016/j.copbio.2005.06.006)

Abstract

With increasing interest in automated synthesis and screening protocols, solid supported chemistry and biochemistry are attractive technologies. Studies with surface-immobilised substrates have been carried out to analyse enzyme accessibility, kinetics and thermodynamics. Several interesting new methods have been developed to monitor enzyme action on substrates attached to a solid phase such as polymer beads glass or gold surfaces. These include fluorescence measurements, MALDI-TOF mass spectrometry, and the use of quartz crystal microbalances to measure weight changes of immobilised molecules directly on the surface. Approaches that allow spatial resolution in single beads have also been reported. The ability of enzymes to reach the inside of beads is becoming better characterised and new supports have been developed that allow improved accessibility. The equilibrium position of reactions on the solid surface can be substantially shifted compared with reactions in solution, and this can be usefully exploited using hydrolases in reverse. Research is also starting to tackle the way in which kinetics are modified when the substrates are surface immobilised.

ORCID iDs

Halling, P.J. ORCID: https://orcid.org/0000-0001-5077-4088

Ulijn, R.V. ORCID: https://orcid.org/0000-0001-7974-3779

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• Item metadata

  Item type:	Article
  ID code:	272
  Dates:	Date Event 2005 Published
  Subjects:	Science > Chemistry
  Department:	Faculty of Science > Pure and Applied Chemistry
  Depositing user:	Users 16 not found.
  Date deposited:	14 Mar 2006
  Last modified:	11 Nov 2024 08:29
  URI:	https://strathprints.strath.ac.uk/id/eprint/272