Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg

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Fontes, N. and Almeida, M.C. and Garcia, S. and Peres, C. and Partridge, J. and Halling, P.J. and Barreiros, S. (2001) Supercritical fluids are superior media for catalysis by cross- linked enzyme microcrystals of subtilisin Carlsberg. Biotechnology Progress, 17 (2). pp. 355-358. ISSN 8756-7938 (http://dx.doi.org/10.1021/bp000148m)

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Abstract

We report on the performance of cross-linked enzyme microcrystals (CLECs) of subtilisin Carlsberg in supercritical fluids (SC-fluids). The catalytic activity of CLECs in SC- ethane was found to be 2- to 10-fold greater than in hexane under the same conditions, using CLECs dried by propanol washing. Air-dried CLECs and lyophilized powders showed much lower activities, reflecting the same hydration hysteresis effects as in organic solvents. Reaction rates were much lower in SC-CO2, especially at higher water activity, probably as a result of acid-base effects of carbonic acid on the enzyme.

ORCID iDs

Fontes, N., Almeida, M.C., Garcia, S., Peres, C., Partridge, J., Halling, P.J. ORCID logoORCID: https://orcid.org/0000-0001-5077-4088 and Barreiros, S.;
CORE (COnnecting REpositories)