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Driving innovations in manufacturing: Open Access research from DMEM

Strathprints makes available Open Access scholarly outputs by Strathclyde's Department of Design, Manufacture & Engineering Management (DMEM).

Centred on the vision of 'Delivering Total Engineering', DMEM is a centre for excellence in the processes, systems and technologies needed to support and enable engineering from concept to remanufacture. From user-centred design to sustainable design, from manufacturing operations to remanufacturing, from advanced materials research to systems engineering.

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Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting

Raddatz, M.S.L. and Dolf, A. and Endl, E. and Knolle, P. and Famulok, M. and Mayer, G. (2008) Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting. Agewandte Chemie-International Edition, 47 (28). pp. 5190-5193. ISSN 1433-7851

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Abstract

In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.