Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting

Raddatz, M.S.L. and Dolf, A. and Endl, E. and Knolle, P. and Famulok, M. and Mayer, G. (2008) Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell sorting. Agewandte Chemie-International Edition, 47 (28). pp. 5190-5193. ISSN 1521-3773 (http://dx.doi.org/10.1002/anie.200800216)

Abstract

In a sophisticated approach to the systematic evolution of ligands by exponential enrichment (SELEX) of cell-type-specific nucleic acid aptamers, the title technique was combined with the use of combinatorial nucleic acid libraries to target a defined subpopulation within composite mixtures of cells (see picture). The ssDNA library after 10 selection cycles bound vital Burkitt lymphoma cells more effectively than the starting library.

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• Item metadata

  Item type:	Article
  ID code:	26096
  Dates:	Date Event June 2008 Published
  Subjects:	Medicine > Other systems of medicine
  Department:	Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
  Depositing user:	Strathprints Administrator
  Date deposited:	20 Aug 2010 15:45
  Last modified:	11 Nov 2024 09:31
  URI:	https://strathprints.strath.ac.uk/id/eprint/26096