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Open Access research with a European policy impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the European Policies Research Centre (EPRC).

EPRC is a leading institute in Europe for comparative research on public policy, with a particular focus on regional development policies. Spanning 30 European countries, EPRC research programmes have a strong emphasis on applied research and knowledge exchange, including the provision of policy advice to EU institutions and national and sub-national government authorities throughout Europe.

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Electrochemical sensing of aptamer-protein interactions using magnetic particle assay and single-use sensor technology

Karadeniz, H. and Erdem, A. and Mayer, G. and Famulok, Michael and Caliskan, A. (2009) Electrochemical sensing of aptamer-protein interactions using magnetic particle assay and single-use sensor technology. Electroanalysis, 21 (11). pp. 1278-1284. ISSN 1040-0397

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Abstract

A magnetic particle assay has been designed herein that can report the interactions of DNA aptamers with their cognate protein targets lysozyme (LYS) and human thrombin (THR). Electrochemical sensing of the biomolecular recognition between each aptamer and its target was explored by using a disposable graphite electrode, PGE, in combination with differential pulse voltammetry (DPV). The magnitudes of the oxidation signals of LYS and THR were measured at +780 mV and +680 mV, respectively, after interaction with the cognate aptamers attached to the surface of magnetic particles. The detection limits estimated for signal to noise ratios above 3.0 correspond to the concentrations of 10.77 μg/mL LYS (769 nM) and 2.00 μg/mL THR (54.5 nM). Our aptamer based approach that combines magnetic particles with a disposable graphite electrode performs well compared to other aptamer-based sensor-formats for quantitative protein detection with respect to sensitivity, selectivity, detection limit, and reproducibility.