Pulse shapes for optimized two-photon laser scanning microscopy

McConnell, G. (2008) Pulse shapes for optimized two-photon laser scanning microscopy. Journal of Physics D: Applied Physics, 41 (20). p. 205502. ISSN 0022-3727 (http://dx.doi.org/10.1088/0022-3727/41/20/205502)

Abstract

A numerical model describing the effect of different pulse shapes on the probability of two-photon absorption for two-photon laser scanning microscopy (2PLSM) is reported. Despite the commercial preference for sech2 pulses, this pulse shape does not provide the best performance. The presented model reveals that a square or parabolic pulse shape is preferable and shows that applications of such pulse shapes can improve the probability of two-photon absorption by up to 50% to facilitate improved 2PLSM.

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• Item metadata

  Item type:	Article
  ID code:	19452
  Dates:	Date Event 21 October 2008 Published
  Subjects:	Medicine > Pharmacy and materia medica Science > Physics
  Department:	Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
  Depositing user:	Strathprints Administrator
  Date deposited:	26 May 2010 13:13
  Last modified:	08 Apr 2024 17:42
  URI:	https://strathprints.strath.ac.uk/id/eprint/19452