Pulse shapes for optimized two-photon laser scanning microscopy

McConnell, G. (2008) Pulse shapes for optimized two-photon laser scanning microscopy. Journal of Physics D: Applied Physics, 41 (20). p. 205502. ISSN 0022-3727

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Abstract

A numerical model describing the effect of different pulse shapes on the probability of two-photon absorption for two-photon laser scanning microscopy (2PLSM) is reported. Despite the commercial preference for sech2 pulses, this pulse shape does not provide the best performance. The presented model reveals that a square or parabolic pulse shape is preferable and shows that applications of such pulse shapes can improve the probability of two-photon absorption by up to 50% to facilitate improved 2PLSM.