Purification of a 5-ht uptake inhibitor from the venom of cerastes vipera

Alzahaby, M. and Harvey, A.L. and Young, L.C. and Faure, G. and Rowan, E.G. (1998) Purification of a 5-ht uptake inhibitor from the venom of cerastes vipera. Toxicon, 36 (4). pp. 601-607. ISSN 1879-3150

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Official URL: http://dx.doi.org/10.1016/S0041-0101(97)00108-6

Abstract

A protein that inhibits the re-uptake of 5-hydroxytryptamine into rat brain synaptosomes was isolated from the venom of the Sahara sand viper (Cerastes vipera) by gel filtration and reverse phase chromatography. It has a molecular weight of 13 739 Da and an 50 of about 50 nM for blocking uptake of 3H-5-HT into rat brain synaptosomes. It also augmented the responses to 5-HT in a smooth muscle preparation. It has phospholipase A2 activity, but it has no lytic activity as measured by its inability to release lactate dehydrogenase from rat brain synaptosomes. Determination of the N-terminal sequence revealed a similarity with a phospholipase A2 previously isolated from Cerastes cerastes venom.

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Item metadata

Item type:	Article
ID code:	17559
Dates:	Date Event April 1998 Published
Keywords:	5-ht uptake inhibitor, venom, cerastes vipera, Pharmacy and materia medica, Toxicology
Subjects:	Medicine > Pharmacy and materia medica
Department:	Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user:	Strathprints Administrator
Date deposited:	06 May 2010 15:44
Last modified:	20 Jan 2021 18:23
URI:	https://strathprints.strath.ac.uk/id/eprint/17559

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