Fluorescence resonance energy transfer from allophycocyanin to malachite green

Rolinski, O.J. and Birch, D.J.S. and McCartney, L.J. and Pickup, J.C. (1999) Fluorescence resonance energy transfer from allophycocyanin to malachite green. Chemical Physics Letters, 309 (5-6). pp. 395-401. ISSN 0009-2614 (http://dx.doi.org/10.1016/S0009-2614(99)00707-1)

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Abstract

The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.

ORCID iDs

Rolinski, O.J. ORCID logoORCID: https://orcid.org/0000-0002-7838-779X, Birch, D.J.S. ORCID logoORCID: https://orcid.org/0000-0001-6400-1270, McCartney, L.J. and Pickup, J.C.;
  • Item type:Article
    ID code:17521
    Dates:
    Date
    Event
    20 August 1999
    Published
    Keywords:fluorescence resonance, energy transfer, allophycocyanin to malachite green, Physics, Physics and Astronomy(all), Physical and Theoretical Chemistry
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Strathprints Administrator
    Date deposited:22 Apr 2010 13:30
    Last modified:20 Jan 2021 18:24
    URI:https://strathprints.strath.ac.uk/id/eprint/17521
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