Protein fluorescence decay : a gamma function description of thermally induced interconversion of amino acid rotamers

Rolinski, O.J. and Scobie, K. and Birch, D.J.S. (2009) Protein fluorescence decay : a gamma function description of thermally induced interconversion of amino acid rotamers. Physical Review E: Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics, 79 (5). 050901. ISSN 2470-0053 (https://doi.org/10.1103/PhysRevE.79.050901)

Abstract

We present a description of fluorescence decay kinetics in complex environments based on gamma functions rather than the conventional approach using exponentials. The gamma function description is tested in measurements on the temperature dependence of the protein human serum albumin (HSA), N-acetyl tryptophanamide (NATA), and 2, 5-dipenyl oxazole (PPO). The monitoring of macromolecular structure and dynamics is demonstrated by means of distinct tryptophan (Trp) rotamer populations and their interconversion in HSA.

ORCID iDs

Rolinski, O.J. ORCID: https://orcid.org/0000-0002-7838-779X

Birch, D.J.S. ORCID: https://orcid.org/0000-0001-6400-1270

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• Item metadata

  Item type:	Article
  ID code:	16950
  Dates:	Date Event 28 May 2009 Published
  Subjects:	Science > Physics
  Department:	Faculty of Science > Physics
  Depositing user:	Strathprints Administrator
  Date deposited:	05 May 2010 15:01
  Last modified:	11 Nov 2024 09:25
  URI:	https://strathprints.strath.ac.uk/id/eprint/16950