Picture of server farm and IT infrastructure

Where technology & law meet: Open Access research on data security & its regulation ...

Strathprints makes available Open Access scholarly outputs exploring both the technical aspects of computer security, but also the regulation of existing or emerging technologies. A research specialism of the Department of Computer & Information Sciences (CIS) is computer security. Researchers explore issues surrounding web intrusion detection techniques, malware characteristics, textual steganography and trusted systems. Digital forensics and cyber crime are also a focus.

Meanwhile, the School of Law and its Centre for Internet Law & Policy undertake studies on Internet governance. An important component of this work is consideration of privacy and data protection questions and the increasing focus on cybercrime and 'cyberterrorism'.

Explore the Open Access research by CIS on computer security or the School of Law's work on law, technology and regulation. Or explore all of Strathclyde's Open Access research...

High-performance liquid chromatographic analysis of amphotericin B in rat plasma using α-naphthol as an internal standard

Italia, J.L. and Singh, D. and Kumar, M.N.V. Ravi (2009) High-performance liquid chromatographic analysis of amphotericin B in rat plasma using α-naphthol as an internal standard. Analytica Chimica Acta, 634 (1). pp. 110-114. ISSN 0003-2670

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

A simple, sensitive and accurate reverse phase high-performance liquid chromatographic (RP-HPLC) method with photo-diode array detector (PDA) was developed and validated for the determination of amphotericin B (AMB) in the rat plasma using a new internal standard (IS) α-naphthol. The plasma samples were subjected to protein precipitation with methanol prior to a HPLC analysis. Chromatographic separations were achieved on a Nucleosil® 100-5C18 (150 mm × 4.6 mm) column. The mobile phase consisted of acetonitrile and sodium acetate buffer (pH 4; 10 mM) in a gradient mode. Detection was carried out at a wavelength of 407 and 294 nm for AMB and IS, respectively. The retention times of AMB and IS were about 6.8 and 7.8 min, respectively. The calibration curve was linear in the range of 10-2000 ng mL−1 for AMB (r2 > 0.998). No significant matrix effect was observed on quantification of AMB or IS. At three quality control concentrations of 20, 500, and 2000 ng mL−1, the intra-day and inter-day relative standard deviation ranged from 1.13% to 4.91%. The limit of detection (LOD) was 5 ng mL−1 and the limit of quantification (LOQ) was 10 ng mL−1 for AMB in rat plasma. This method is simple, sensitive, rapid and does not require any extensive sample purification before injecting into HPLC.